| fedbatch_demo | Simple fedbatch model for illustration of common principles of a fedbatch process. |
| fedbatch_Cmole | Fedbatch simulation based on the use of stoichiometric
analysis (C- and degree-of-reduction balancing) to improve the simple
model in demo_fedbatch. In this way also the CO2 production is obtained and RQ can be calculated. |
| fedbatch_kla | Includes a common model for KLa as function of RPM and aeration, which makes DOT dependent on these control parameters. |
| fedbatch_ecoli | An overflow metabolism model is used to simulate the acetate formation/consumption by E. coli W3110 in a process started as batch, followed by first exponential feed to resorb the acetate and then constant feed to reach high cell density. |
| yeast_fedbatch | This simulation utilizes exponential feed to control
ethanol production by overflow metabolism in a fed-batch culture of S.
cerevisiae. The model used is described in the SIMSPEC file yeast_model. |
| chemostat_demo | Simulation of steady-state values of concentrations in a chemostat as function of dilution rate. |
| chemostat_dyn | Dynamic simulation of a chemostat. Illustrates how to start and control a chemostat. |
| yeast_chemostat_ss | This simulation shows steady state concentrations of cells, glucose, ethanol, and DOT in a S. cerevisiae chemostat. |
| chemostat_mutant | Dynamic simulation that illustrates take-over by a
single plasmid free cell at the start of a continuous culture of a
recombinant protein producer. |
| monod_inhibition | This simulation is used to illustrate different inhibition types (competitive, non-competitive, etc.) in Monod plots. |
| yeast_model | The overflow model used in the yeast simulations above is
illustrated as Monod plots of specific rates of glucose and oxygen
uptake and cell and ethanol production. |
| exp_growth | Shows how mu-max determines the maximum cell density obtained in an (aerobic) batch bioreactor (at constant KLa). |
| Sconsumption | Illustrates how fast the limiting substrate is exhausted in a fed-batch if the feed is interrupted. |
| Sgradient_bead | This application simulatesthe substrate concentration profile in a
homogeneous bead/floc at different concentration of S and biocatalyst. The effectiveness factor is also calculated for the bead. |
| HTST_sterilisation | The sterilisation time is calculated as function of temperature in a continuous sterilisation process. |
| Batch_sterilisation | The lethal rate and accumulated sterilisation dose is calculated during a batch autoclaving process. |
| Astat | This file simulates an accelerostate, i.e. a pseudo-chemostat in which the dilution rate is constantly increased or reduced with time. |
| yeast_Astat | The Astat simulation is applied on a S. cerevisiae culture. |
|
The models used in the simulations above assume that oxygen does not
become limiting. If process control settings result in oxygen
limitation the results may become completely wrong. Most fed-batch
models above are based on parameters typical for E. coli without
overflow metabolism (as if grown on glycerol). Some of the applications above include links to student exercises. |